Catalog Number - 65308-10P-2

Zx Similars C8™ Octyl is a traditional reversed-phase HPLC stationary phase manufactured on high-purity Type-B spherical silica with a fully endcapped octyl (C8) bonded phase. Designed as a cost-effective alternative to Agilent Zorbax® C8 columns, it offers comparable chromatographic behavior for USP L7 methods while providing the moderate hydrophobic retention, reproducibility, and reliability expected from a conventional C8 stationary phase. Compared to C18 columns, C8 phases generally provide reduced retention and faster elution of hydrophobic analytes.

65308-10P-2 is a 100 mm x 2.1 mm ID narrow-bore analytical column packed with 3 µm Zx Similars C8 particles. The 100 mm length provides an excellent balance of chromatographic resolution and analysis speed, while the 2.1 mm ID format minimizes solvent consumption and is particularly well suited for LC-MS applications. The 3 µm particle size delivers higher efficiency and improved peak capacity than conventional 5 µm columns, although it generates increased operating pressure. Typical operating flow rates are approximately 0.2–0.3 mL/min and require a system capable of reliable low-flow operation with low-volume plumbing and flow cells.

This USP L7 column is ideal for hydrophobic and neutral compounds when less retention is desired than a comparable C18 phase. Its narrow-bore design, low-flow operation, and enhanced efficiency make it a popular choice for LC-MS methods, pharmaceutical analysis, long-chain fatty acid separations, and high-sensitivity analytical workflows requiring chromatographic behavior similar to Agilent Zorbax® C8 columns.

Key Benefits

• Type-B spherical silica stationary phase

• Suitable alternative for USP L7 methods

• Comparable selectivity to Agilent Zorbax® C8 columns

• Narrow-bore format ideal for LC-MS applications

• Balanced speed and resolution with 100 mm length

• Higher efficiency than comparable 5 µm columns

• Reduced solvent consumption

• End-capped C8 bonded phase

Typical Applications

• USP L7 methods

• LC-MS analysis

• Reversed-phase HPLC

• Pharmaceutical analysis

• Long-chain fatty acid analysis

• Quality control testing

• Method development

• Method transfer and validation

Specifications

• Product Type: HPLC Column

• Stationary Phase: Zx Similars C8 Octyl

• USP Classification: L7

• Particle Size: 3 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: C8 (Octyl)

• Endcapped: Yes

• Column Dimensions: 2.1 mm ID x 100 mm

• Length: 100 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Narrow-bore format optimized for LC-MS applications

• Requires an HPLC or LC-MS system capable of reliable low-flow operation

• Higher efficiency than comparable 5 µm columns

• Increased operating pressure compared to larger particle sizes

• Reduced solvent consumption compared to 4.6 mm ID columns

• Provides less retention than comparable C18 stationary phases

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